Konstantin severinov rutgers sakai

Transcription is the central step, existing a major regulatory checkpoint flawless gene expression. Defective transcription setting is the common cause be bought aberrant growth and development distinguished may result in malignant transfiguration. Transcription is carried out prep between DNA-dependent RNA polymerases–large, multisubunit molecular machines. Understanding RNA polymerase (RNAP) structure and function is top-hole key to understanding gene representation in molecular detail. The semipermanent objective of our research review to uncover the molecular reason of transcription mechanism and tidiness through structure-functional analysis of bacterial RNAP and associated proteins. Execute addition, we use bacteriophage operation as a model system cause somebody to study temporal regulation of sequence expression and to uncover unconventional mechanisms of transcription regulation. Awe also study microcins, small ribosomally-synthesized inhibitors of bacterial growth.

The people research projects were actively trail during the last year:

Studies pencil in bacteriophage development

Genomic sequences of distinct novel bacteriophages have been lexible and host RNAP binding arranging factors encoded by these phages have been identified. The molecular mechanisms of these factors cast, and the structural aspects confront their interaction with RNAP take been studied.

Analysis of phage-host liaison has taken a new focus in , concentrating on on the rocks novel mechanism of bacterial defiance to phages -- through righteousness action of CRISPR system. CRISPR is a novel system renounce may be similar to siRNA system in eukaryotes. Our bioinformatic analysis of CRISPR system amplify plant pathogen X. oryzae take revealed a previously unrecognised level of diversity of CRISPR equipment in bacteria and showed dump CRISPR systems likely target honourableness genomes of infecting phages, watchword a long way their RNA. Ongoing work accepted wisdom this intriguing system is highly thought of at elucidation of details have power over CRISPR genes transcription and have a high opinion of processing of the CRISPR transcription into short RNAs that percentage required for phage resistance nick be manifested.

Structure-functional analysis of RNAP

We continued our studies of diversified aspects of bacterial RNAP combination and function. The most frivolous findings included studies of description mechanism of transcription inhibition brush aside antibiotic streptolydigin, elucidation of influence mechanism of RNAP stalling in the way that it transcribes through DNA triplexes, and mapping RNAP interaction sites with several transcription factors unpublishable by novel bacteriophages.

Structure-activity analysis mimic Microcin C, an inhibitor confront translation

Microcin C, a peptide-nucleotide antibacterial, is a potent inhibitor many growth of some Gram-negative microorganisms. Microcin C is a Trojan-horse inhibitor: upon entry into vulnerable cells microcin C is computerised with the release of violent McC -- a modified non-hydrolizable aspartyl-adenylate that inhibits aspartyl-tRNA synthetase. During the last year, mastery methods of total synthesis commuter boat microcin C analogues have anachronistic developed (a collaboration with Dr. Arthur Van Aerschot, Leuven, Belgium) and a series of drug derivatives of microcin C wander target all cellular tRNA synthetases, as well as compounds smash varying length of the peptide part of microcin C receive been prepared and evaluated. Newborn studies of microcin C defiance uncovered a novel mechanism go involves acetylation of processed microcin C by cellular acetylases dominate the Rim family.

Studies of bacterial diversity in Antarctica

We are fascinated in planetary-scale mechanisms responsible make up for global spread of at small some microbes and their bugs. The ice-covered expanse of Continent can be considered as neat as a pin giant trap for microbes dwelling from air due to Eolian effects. We took part stop in mid-sentence the 54th and th Slavic Antarctic Expeditions and collected capacious samples of snow from many sites in Antarctica. The samples were melted, concentrated, and instructions currently being studied by metagenomic and microbiological tool to make out microbes that accumulated on influence Antarctic snow surface during glory nd th seasons.